To gain insight into the transmission of tuberculosis (TB)
in peri-urban Kampala-Uganda, we performed a household contact study using
children as a surrogate for recent transmission ofMycobacterium tuberculosis (MTB). Using this approach, we sought to
understand M. tuberculosiscomplex (MTBC) lineage diversity,
distribution and how these relate to TB transmission to exposed children.
MTBC isolates from children aged ≤ 15 years, collected from 2002 to 2010 in a household-contact
study, were analyzed using a LightCycler RT-PCR SNP genotyping assay (LRPS).
The resultant genotypic data was used to determine associations between MTBC
lineage and the children’s clinical and epidemiological characteristics.
Of the 761 children surveyed, 9 % (69/761) had
culture-positive TB an estimate in the range of global childhood TB; of these
71 % (49/69) were infected with an MTBC strain of the “Uganda family”,
17 % (12/69) infected with MTBC lineage 4 strains other than MTBC Uganda
family and 12 % (8/69) infected with MTBC lineage 3, thereby
disproportionately causing TB in the study area. Overall the data showed no
correlation between the MTBC lineages studied and transmission (OR = 0.304; P-value = 0.251;
CI: 95 %; 0.039-2.326) using
children a proxy for TB transmission.
Our findings indicate that MTBC Uganda family strains are
the main cause of TB in children in peri-urban Kampala. Furthermore, MTBC
lineages did not differ in their transmissibility to children.
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By:
Makerere University
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