The pathogenesis of sinonasal polyposis remains unclear, in
spite of several investigative approaches. Antrochoanal polyps, a subgroup of
sinonasal polyposis along with allergic- and chronic-inflammatory nasal polyps,
mostly originate from the maxillary sinus and develop as a unilateral,
pedunculated mass towards the nasopharynx. The human papillomavirus (HPV) is
discussed as a possible causative and influencing factor in development and
progression of sinonasal polyposis. This study aims to elucidate HPV frequency
in nasal polyps and antrochoanal polyps.
Genomic DNA from 257 tissue specimens (166 nasal polyps, 39
antrochoanal polyps and 52 nasal turbinates) was subjected to three different
established HPV- polymerase chain reaction assays, testing for 37 low- and
high-risk HPV. In addition, immunohistochemical analyses for HPV16 were carried
out, as well as immunohistochemistry and western blots of p16, a biomarker for
HPV induced cancer.
HPV-DNA was detected in 53.8% of antrochoanal polyps, 15.1%
of nasal polyps, and 5.8% of nasal turbinates. HPV16 was the predominant type
with a detection rate of 76% in nasal polyps and 62% in antrochoanal polyps.
Immunohistochemically, HPV positive tissues stained positive for HPV16 antigens
and p16 in epithelial cell layers. No significant p16 overexpression was traceable
in antrochoanal polyps, nasal polyps and nasal turbinates by western blot.
There was no correlation of HPV-status with sex, age, smoking, alcohol
consumption or allergic background.
The present study shows a significant frequency of high-risk
type HPV16 in antrochoanal polyps. Absence of oncogenic transformation or
correlation of the HPV-status with clinical data suggests a latent
superinfection, possibly because of anatomical proximity to the oropharynx.
Below: HPV Immunohistochemistry.
Below: HPV Immunohistochemistry.
IHC Overview of a representative HPV-positive antrochoanal
polyp with HPV16 staining (A). HPV16 E6 protein staining of a representative
HPV-positive antrochoanal polyp, relevant epithelial region shown (B) with
corresponding negative control (C). HPV16 E6 protein staining of a
representative HPV-positive nasal polyp, relevant epithelial region shown and
subepithelial stained cells (D) with corresponding negative control (E).
Discontinuous epithelial cell clusters stained positive in HPV-positive
antrochoanal- and nasal polyps.
Full article at: http://goo.gl/kU4LVr
By:
Margaret McLaughlin-Drubin, Editor
1Experimental Otorhinolaryngology,
ENT-Hospital, Head and Neck Surgery, Friedrich-Alexander University
Erlangen-Nürnberg (FAU), Erlangen, Germany
2Department of Pathology,
Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany
Brigham and
Women's Hospital/Harvard Medical School, UNITED STATES
Competing Interests: The authors have declared
that no competing interests exist.
Conceived and designed the experiments: JZ OW. Performed the
experiments: MK OW. Analyzed the data: KT AA OW MK. Contributed
reagents/materials/analysis tools: JZ OW AA. Wrote the paper: MK OW.
* E-mail: ed.negnalre-ku@reldnew.falo (OW); Email: ed.zniam-nizideminu@reonk.ekieram (MK)
More at: https://twitter.com/hiv_insight
No comments:
Post a Comment