Introduction:
Host
colonization by Candida species is an
important predisposing factor to candidiasis, which seems to be more frequent
in human immunodeficiency virus (HIV)-infected patients. Knowledge about the
distribution, antifungal susceptibility, and virulence of oral Candida isolates is
important for effective management of candidiasis.
Methodology: Oral rinses were
collected from 242 HIV-infected patients without clinical evidence of
candidiasis seen at the AIDS referral center in Londrina, Brazil. Species were
identified by standard phenotypic and molecular methods, and characterized in vitro according to
antifungal susceptibility, cell surface hydrophobicity, biofilm formation, and
enzyme activities.
Results:
Oral Candida colonization
was detected in 50.4% of patients and combined use of antiretroviral therapy
and protease inhibitor had a protective effect against colonization. Candida albicans (75.2%) was the
most prevalent species. A high proportion of Candida spp. (39.9%)
showed decreased susceptibility to fluconazole. Five isolates were resistant to
nystatin. Protease and phospholipase activities were detected in 100% and 36.8%
of isolates, respectively. Most isolates displayed a hydrophobic property that
was associated with biofilm formation ability.
Conclusions:
A significant
number of oral Candida species
exhibiting decreased susceptibility to fluconazole were isolated from colonized
HIV-infected individuals. Furthermore, all isolates expressed potential
virulence attributes in vitro. Given the high incidence and severity of fungal
infections in HIV-infected individuals, the results of this study reinforce the
importance of anti-fungal susceptibility testing, which contributes to
therapeutic strategies and highlights the need for continuous surveillance of Candida colonization in
this population.
Full article at: http://goo.gl/PC3HxV
By: Suelen Balero de Paula, Alexandre Tadachi Morey, Jussevania
Pereira Santos, Pollyanna M. C. dos Santos, Danielle G. Gameiro, Gilselena
Kerbauy, Ester M. Sena, Luiz T. Ueda, Marcelo Carneiro, Phileno Pinge-Filho,
Lucy Megumi Yamauchi, Sueli Fumie Yamada-Ogatta
Universidade
Estadual de Londrina, Londrina, Paraná (PR), Brasil
doi:10.3855/jidc.6970
More at: https://twitter.com/hiv
insight
And: http://twitter.com/Prison
Health
No comments:
Post a Comment