Due to the increasing rates of oropharyngeal cancer, oral
HPV infection is a significant concern. Methods for detecting oral HPVs is not
standardized as there are different techniques available. We propose that use
of oral rinse samples to detect for HPVs is a suitable technique within a
clinic setting. Thus, our main objective is to study HPV detection in oral
rinse samples.
In our study, we used oral rinse sample collection coupled
with real-time PCR to detect for HPVs types 16 and 18, and preferentially
amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal
squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients.
Thirty three percent of 100 cancer patients were positive
for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy
controls was positive (this subject was positive for HPV18).
Our results indicate that HPV detection in oral rinse
samples may be useful as a screening tool to detect HPV-associated oral
cancers.
Below: FAP PCR results on an electrophoresis gel. An expected band of ~480 bp indicates an HPV positive sample
Below: Comparison of HPV16 viral load (copies/ng DNA) between the two cancer groups. No statistically significant difference was seen (p = 0.40). The gray boxes demonstrate range from lower to upper quartiles. The median is represented by a horizontal line. Maximum and minimum values are indicated by the vertical lines. Viral load variation between each group was demonstrated. The median viral load in OPSCC was slightly higher than non-OPSCC cases
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at:
1Oral Health Sciences, University of
Washington, 1959 NE Pacific St., Seattle 98195, WA, USA
2Seattle Cancer Care Alliance, 825 Eastlake
Ave E, Seattle 98109, WA, USA
3Pathology, University of Washington,
Seattle, WA, USA
4Harborview Medical Center, 908 Jefferson,
Seattle 98104, WA, USA
5University of Washington, 1959 NE Pacific
St., Seattle 98195, WA, USA
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