BACKGROUND:
Chlamydia
trachomatis (C. trachomatis), an obligate intracellular bacterium, is the
commonest infectious bacterial agent of sexual transmission throughout the
world. It has been shown that the presence of this bacteria in the cervix
represents a risk regarding HPV persistence and, thereafter, in developing
cervical cancer (CC). Prevalence rates may vary from 2% to 17% in asymptomatic
females, depending on the population being analysed. This study reports the
identification of C. trachomatis in a cohort of 219 HPV-infected Colombian
females.
METHODS:
C.
trachomatis infection frequency was determined during each of the study's
follow-up visits; it was detected by amplifying the cryptic plasmid sequence by
polymerase chain reaction (PCR) using two sets of primers: KL5/KL6 and KL1/KL2.
Infection was defined as a positive PCR result using either set of primers at
any time during the study. Cox proportional risk models were used for
evaluating the association between the appearance of infection and a group of
independent variables.
RESULTS:
Base line
C. trachomatis infection frequency was 28% (n = 61). Most females infected by
C. trachomatis were infected by multiple types of HPV (77.42%), greater
prevalence occurring in females infected with HPV-16 (19.18%), followed by
HPV-58 (17.81%). It was observed that females having had the most sexual
partners (HR = 6.44: 1.59-26.05 95%CI) or infection with multiple types of HPV
(HR = 2.85: 1.22-6.63 95%CI) had the greatest risk of developing C. trachomatis.
CONCLUSIONS:
The
study provides data regarding the epidemiology of C. trachomatis /HPV
coinfection in different population groups of Colombian females and contributes
towards understanding the natural history of C. trachomatis infection.
Below: Percentage of females infected by C. trachomatis per visit
Below: The probability of the risk of the women in this cohort acquiring C. trachomatis infection as time elapsed
Below: The time taken to clear Chlamydia trachomatis infection in the cohort of females initially infected by HPV and C. trachomatis
By: Quinónez-Calvache EM1,2, Ríos-Chaparro DI3, Ramírez JD3, Soto-De León SC1,4, Camargo M1,4, Del Río-Ospina L1,5, Sánchez R1,5, Patarroyo ME1,5,Patarroyo MA1,4.
- 1Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
- 2Biotechnology Institute, Faculty of Sciences, Universidad Nacional de Colombia, Bogotá, Colombia.
- 3Grupo de Investigaciones Microbiológicas-UR (GIMUR), Faculty of Natural and Mathematical Sciences, Universidad del Rosario, Bogotá, Colombia.
- 4School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Colombia.
- 5School of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia.
- PLoS One. 2016 Jan 25;11(1):e0147504. doi: 10.1371/journal.pone.0147504. eCollection 2016.
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