Human papillomaviruses (HPVs)
have previously been reported to infect epithelial trophoblast cells of the placenta.
To investigate this possibility, 200 placental samples from Zambian women were separated
into HIV+ and HIV- groups and tested for HPV by redundant primer PCR,
using GP5+/GP6+ and CPI/CPII primer sets.
Three HPV genotypes (HPV6, 16 and 90)
were detected in placental samples. Whereas, 20 different HPV genotypes were detected
in vaginal sampling of the same patients, suggesting that compartment specific sub-populations
of HPV may exist. The incidence of HPV16 in placental samples was almost 2-fold
greater in HIV+ women compared to HIV- (p = 0.0241). HPV16 L1 expression, detected by immunochemistry,
was significantly higher in HIV+ than HIV- samples (p = 0.0231). HPV16 DNA was detected in the
nuclei of trophoblast cells by in situ hybridization.
Overall, these results suggest that HPVs infect the placenta and that HIV significantly
influences these infections.
Below: The distribution of HPV in
HIV-negative and HIV-positive placental samples. Three types of HPVs were
isolated including the low risk (LR)-HPV6, high-risk (HR)-HPV16 and the rarely
reported HPV90. The pie-chart on the left side shows the distribution of HPV
genotypes in HIV-negative placental tissues and; The pie-chart on the right
side shows the distribution of HPV genotypes in HIV-positive placental tissues.
To determine the HPV genotypes, the PCR products were cloned into the pGEMT
vector and sequenced. There was a significant difference (p < 0.05; p =
0.0241) in the distribution of HPV16 between the HIV−/HPV16+ and the
HIV+/HPV16+ groups, with an odds ratio of 2.1. There was no significant
difference (p > 0.05; p = 0.0864) in the distribution of HPV6 between the
HIV−/HPV16+ and the HIV+/HPV16+ groups.
Full article at: http://goo.gl/1bnO5l
By:
- 1Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
- 2University of Miami Miller School of Medicine, Miami, FL, USA.
More at: https://twitter.com/hiv insight
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