Soluble cervicovaginal
biomarkers of inflammation, immune activation and risk of HIV acquisition are
needed to reliably assess the safety of new biomedical prevention strategies
including vaccines and microbicides. However, a fuller understanding of
expression profiles in women at high risk for HIV infection is crucial to the
effective use of these potential biomarkers in Phase 3 trial settings.
We have measured 45 soluble proteins and peptides in cervicovaginal lavage samples from 100 HIV negative women at high risk for HIV infection. Women were followed over one menstrual cycle to investigate modulation by hormonal contraception, menstrual cycle phase, recent sexual exposure and intravaginal practices.
Women using injectable DMPA had increased concentration of several soluble proteins of the innate and adaptive immune system, including IL-1α, IL-1β, IL-2, MIP-1β, IP-10, IL-8, TGF-β, HBD4, IgA, IgG1, and IgG2. Women using combined oral contraceptives had a similar signature. There were differences in concentrations among samples from post-ovulation compared to pre-ovulation, notably increased immunoglobulins. Increased prostate-specific antigen, indicative of recent sexual exposure, was correlated with increased IL-6, MCP-1, and SLPI, and decreased GM-CSF and HBD3.
The identified signature profiles may prove critical in evaluating the potential safety and impact on risk of HIV acquisition of different biomedical intervention strategies.
We have measured 45 soluble proteins and peptides in cervicovaginal lavage samples from 100 HIV negative women at high risk for HIV infection. Women were followed over one menstrual cycle to investigate modulation by hormonal contraception, menstrual cycle phase, recent sexual exposure and intravaginal practices.
Women using injectable DMPA had increased concentration of several soluble proteins of the innate and adaptive immune system, including IL-1α, IL-1β, IL-2, MIP-1β, IP-10, IL-8, TGF-β, HBD4, IgA, IgG1, and IgG2. Women using combined oral contraceptives had a similar signature. There were differences in concentrations among samples from post-ovulation compared to pre-ovulation, notably increased immunoglobulins. Increased prostate-specific antigen, indicative of recent sexual exposure, was correlated with increased IL-6, MCP-1, and SLPI, and decreased GM-CSF and HBD3.
The identified signature profiles may prove critical in evaluating the potential safety and impact on risk of HIV acquisition of different biomedical intervention strategies.
Below: Distribution of analyte concentrations in cervicovaginal lavage samples for 370 healthy visits
Full article at: http://goo.gl/QLQAIc
By:
Suzanna C. Francis, Kathy Baisley, Trong T. Ao, Saidi
Kapiga, Richard J. Hayes
MRC Tropical Epidemiology Group, London School of Hygiene
and Tropical Medicine, London, United Kingdom
Suzanna C. Francis, Deborah Watson-Jones, Trong T. Ao,
Kaballa Maganja, Aura Andreasen, Saidi Kapiga
Mwanza Intervention Trials Unit, National Institute for
Medical Research, Mwanza, Tanzania, United Republic of Tanzania
Yanwen Hou, Gary R. Coulton
Division of Basic Medical Sciences, St. George's Medical
School, University of London, London, United Kingdom
Janneke van de Wijgert
Institute of Infection and Global Health, University of
Liverpool, Liverpool, United Kingdom
Carolina Herrera, Robin J. Shattock
Mucosal Infection and Immunity Group, Imperial College,
Department of Medicine, London, United Kingdom
Deborah Watson-Jones, Aura Andreasen
Clinical Research Department, London School of Hygiene and
Tropical Medicine, London, United Kingdom
More at: https://twitter.com/hiv insight
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